#### calibration curve

```I am setting up a chemistry calibration curve using data below

..020                   0
..045                   10
..245                   40  etc

Plotting in XY scatter chart and get trendline using polynomial function.
How can I determine the  concentration of an unkown solution ? I want to use
trendline data to do this.

```
 0
pfgnj (2)
2/17/2006 1:05:21 PM
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```Put the Conc data in column A and the OD data in B
You can have headers ("Concentration", "OD" ) in row 1
Let's say the last row is 10
Select A1:B10, click the chart tool, select XY chart, use the first subtype
(markers without line)
On chart right click on a data point, select Insert Trendline and specify
polynomial or required order
To out the coefficients into cell use LINEST as shown in
http://www.stfx.ca/people/bliengme/ExcelTips/Polynomial.htm

You now have equation such as y = ax^2 + bx +c where x is conc and y is OD
You will have real numbers 12.45 = 2.34c^2 - 5.46c + 8.9 and will need to
solve for x

Most calibration curve I every used were linear y =mx + b
So x = (y - b)/m

best wishes
--
Bernard V Liengme
www.stfx.ca/people/bliengme
remove caps from email

"PAUL GRAZIDE" <pfgnj@worldnet.att.net> wrote in message
news:lSjJf.34500\$id5.1164@bgtnsc04-news.ops.worldnet.att.net...
>I am setting up a chemistry calibration curve using data below
>
> .020                   0
> .045                   10
> .245                   40  etc
>
> Plotting in XY scatter chart and get trendline using polynomial function.
> How can I determine the  concentration of an unkown solution ? I want to
> use trendline data to do this.
>

```
 0
bliengme5824 (3040)
2/17/2006 1:22:32 PM
```Are you sure that a polynomial is an appropriate calibration curve here?  You
don't give enough points to assess the fit, but I am much more familiar with
OD calibration curves that are either log-linear or a 4- or 5-parameter
logistic equation.

Jerry

"PAUL GRAZIDE" wrote:

> I am setting up a chemistry calibration curve using data below
>
> ..020                   0
> ..045                   10
> ..245                   40  etc
>
> Plotting in XY scatter chart and get trendline using polynomial function.
> How can I determine the  concentration of an unkown solution ? I want to use
> trendline data to do this.
```
 0
2/17/2006 10:40:16 PM
```Polynomial in this case is correct, because the curve is not linear.  If
curve was linear, then i would use y=mx+b.

Thanks

"Jerry W. Lewis" <post_a_reply@no_e-mail.com> wrote in message
news:730C2895-7577-42FF-A670-99BA4441E2CC@microsoft.com...
> Are you sure that a polynomial is an appropriate calibration curve here?
> You
> don't give enough points to assess the fit, but I am much more familiar
> with
> OD calibration curves that are either log-linear or a 4- or 5-parameter
> logistic equation.
>
> Jerry
>
> "PAUL GRAZIDE" wrote:
>
>> I am setting up a chemistry calibration curve using data below
>>
>> ..020                   0
>> ..045                   10
>> ..245                   40  etc
>>
>> Plotting in XY scatter chart and get trendline using polynomial function.
>> How can I determine the  concentration of an unkown solution ? I want to
>> use
>> trendline data to do this.

```
 0
pfgnj (2)
2/17/2006 11:26:32 PM
```I did not suggest that your data was not curved.  I suggested that over an
appropriate range log(OD) might straighten it into a line.  Barring that, you
would probably get a better fit from a model that flattens out toward max and
min values (as OD must) instead of increasing or decreasing without bound (as
a polynomial must).

Jerry

"PAUL GRAZIDE" wrote:

> Polynomial in this case is correct, because the curve is not linear.  If
> curve was linear, then i would use y=mx+b.
>
> Thanks
>
>
> "Jerry W. Lewis" <post_a_reply@no_e-mail.com> wrote in message
> news:730C2895-7577-42FF-A670-99BA4441E2CC@microsoft.com...
> > Are you sure that a polynomial is an appropriate calibration curve here?
> > You
> > don't give enough points to assess the fit, but I am much more familiar
> > with
> > OD calibration curves that are either log-linear or a 4- or 5-parameter
> > logistic equation.
> >
> > Jerry
> >
> > "PAUL GRAZIDE" wrote:
> >
> >> I am setting up a chemistry calibration curve using data below
> >>
> >> ..020                   0
> >> ..045                   10
> >> ..245                   40  etc
> >>
> >> Plotting in XY scatter chart and get trendline using polynomial function.
> >> How can I determine the  concentration of an unkown solution ? I want to
> >> use
> >> trendline data to do this.
>
>
>
```
 0
2/18/2006 2:32:26 AM

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